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    An electrochemical deposition method was used to fabricate a gold nanoflower (AuNF) and carbon nanoparticle (CNP) modified carbon paper (CP) sensor (AuNFs-CNPs/CP) for the low-cost detection of 5-methyltetrahydrofolate (5-mTHF) in egg yolk. AuNF morphology and structures were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS), revealing nanoflower sizes in the 50 to 200 nm range. AuNFs formed on the sensor were in the Au0. We evaluated 5-mTHF assay performance using cyclic voltammetry, differential pulse voltammetry and chronoamperometry. The AuNFs-CNPs/CP sensor detected 5-mTHF concentrations in the ranges from 1 to 5 mg L-1 and 1-20 μg L-1, with an excellent limit of detection of 1 μg L-1 and good selectivity toward 5-mTHF, when compared to other potentially interfering molecules in samples. The AuNFs-CNPs/CP sensor was also used to detect 5-mTHF in folate-rich, and was found to be twice than that of ordinary egg yolk. Copyright © 2020. Published by Elsevier Ltd.


    Yanan Yu, Dandan Sun, Yuning Liu, Qingyu Zhao, Yuchang Qin, Junmin Zhang. A novel electrochemical paper sensor for low-cost detection of 5-methyltetrahydrofolate in egg yolk. Food chemistry. 2021 Jun 01;346:128901

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    PMID: 33450645

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