Correlation Engine 2.0
Clear Search sequence regions

  • endothelium (1)
  • ester (2)
  • humans (2)
  • hydrolases (2)
  • lipoprotein (25)
  • lipoproteins hdl (2)
  • mass (1)
  • mice (3)
  • PON1 (13)
  • pon1 protein human (1)
  • protein human (1)
  • serum (6)
  • Sizes of these terms reflect their relevance to your search.

    Endothelial lipase (EL) is a strong modulator of the high-density lipoprotein (HDL) structure, composition, and function. Here, we examined the impact of EL on HDL paraoxonase 1 (PON1) content and arylesterase (AE) activity in vitro and in vivo. The incubation of HDL with EL-overexpressing HepG2 cells decreased HDL size, PON1 content, and AE activity. The EL modification of HDL did not diminish the capacity of HDL to associate with PON1 when EL-modified HDL was incubated with PON1-overexpressing cells. The overexpression of EL in mice significantly decreased HDL serum levels but unexpectedly increased HDL PON1 content and HDL AE activity. Enzymatically inactive EL had no effect on the PON1 content of HDL in mice. In healthy subjects, EL serum levels were not significantly correlated with HDL levels. However, HDL PON1 content was positively associated with EL serum levels. The EL-induced changes in the HDL-lipid composition were not linked to the HDL PON1 content. We conclude that primarily, the interaction of enzymatically active EL with HDL, rather than EL-induced alterations in HDL size and composition, causes PON1 displacement from HDL in vitro. In vivo, the EL-mediated reduction of HDL serum levels and the consequently increased PON1-to-HDL ratio in serum increase HDL PON1 content and AE activity in mice. In humans, additional mechanisms appear to underlie the association of EL serum levels and HDL PON1 content.


    Irene Schilcher, Julia T Stadler, Margarete Lechleitner, Andelko Hrzenjak, Andrea Berghold, Gudrun Pregartner, Marie Lhomme, Michael Holzer, Melanie Korbelius, Florian Reichmann, Anna Springer, Christian Wadsack, Tobias Madl, Dagmar Kratky, Anatol Kontush, Gunther Marsche, Saša Frank. Endothelial Lipase Modulates Paraoxonase 1 Content and Arylesterase Activity of HDL. International journal of molecular sciences. 2021 Jan 13;22(2)

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 33450841

    View Full Text