Evaluating cytokine production by flow cytometry using brefeldin A in mice.

Stephen B Kovacs, Changhoon Oh, Youssef Aachoui, Edward A Miao

STAR protocols 2021 Mar 19

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Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of interest. To overcome this, we utilize injection of brefeldin A into mice to enable flow cytometric analysis of in situ cytokine production during a bacterial infection. While we evaluate IFN-γ production during Burkholderia thailandensis infection, this protocol can be applied to other cytokines and other mouse models. For complete details on the use and execution of this protocol, please refer to Kovacs et al. (2020) and Liu and Whitton (2005). © 2020 The Authors.

Citation

Stephen B Kovacs, Changhoon Oh, Youssef Aachoui, Edward A Miao. Evaluating cytokine production by flow cytometry using brefeldin A in mice. STAR protocols. 2021 Mar 19;2(1):100244