Rice Haploid Inducer Development by Genome Editing.

Juntao Liu, Dawei Liang, Li Yao, Ya Zhang, Chunxia Liu, Yubo Liu, Yanli Wang, Hongju Zhou, Timothy Kelliher, Xingping Zhang, Anindya Bandyopadhyay

Methods in molecular biology (Clifton, N.J.) 2021

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The current method to induce haploids in rice is anther culture, which is time-consuming and labor intensive and only works for some varieties. Here we describe a seed-based haploid induction system created by CRISPR/Cas9 technology. By editing OsMATL, we generate rice haploid inducer lines with a 2-5% haploid induction rate in different germplasms.

Citation

Juntao Liu, Dawei Liang, Li Yao, Ya Zhang, Chunxia Liu, Yubo Liu, Yanli Wang, Hongju Zhou, Timothy Kelliher, Xingping Zhang, Anindya Bandyopadhyay. Rice Haploid Inducer Development by Genome Editing. Methods in molecular biology (Clifton, N.J.). 2021;2238:221-230