STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing.

Kana Aoki, Shota Harada, Keita Kawaji, Kenji Matsuzawa, Seiichi Uchida, Junichi Ikenouchi

Nature communications 2021 Jan 20

filter terms:

The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.

Citation

Kana Aoki, Shota Harada, Keita Kawaji, Kenji Matsuzawa, Seiichi Uchida, Junichi Ikenouchi. STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing. Nature communications. 2021 Jan 20;12(1):480