Mycobacterium szulgai as positive control helps to detect contaminations in the detection of Mycobacterium tuberculosis-complex in formalin fixed, paraffin embedded tissues by 16SrDNA PCR].

Torsten Goldmann, Doris Hillemann, Florian Maurer, Barbara Kalsdorf, Rosemarie Krupar, Florian Stellmacher, Sven Perner

Der Pathologe 2021 Feb

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The detection of Mycobacterium tuberculosis complex DNA by PCR using formalin-fixed paraffin-embedded material has become an integral part of molecular-pathological diagnostics. We describe an approach that enables the detection of contamination by using Mycobacterium szulgai as a positive control, contributing to the reduction of false-positive results.

Citation

Torsten Goldmann, Doris Hillemann, Florian Maurer, Barbara Kalsdorf, Rosemarie Krupar, Florian Stellmacher, Sven Perner. Mycobacterium szulgai as positive control helps to detect contaminations in the detection of Mycobacterium tuberculosis-complex in formalin fixed, paraffin embedded tissues by 16SrDNA PCR]. Der Pathologe. 2021 Feb;42(1):83-85