Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis.

Fenretinide is a synthetic retinoid pharmaceutical linked to ceramide build-up in vivo. Saposin D is an intralysosomal protein necessary for ceramide binding/degradation. We show, via electronic absorption spectroscopy, fluorescence spectroscopy, and ceramide hydrolysis assays, that fenretinide is bound by saposin D {K a = (1.45 ± 0.49) × 105 M-1}, and affects ceramide solubilization/degradation. This journal is © The Royal Society of Chemistry 2020.

Citation

Brandon T Milliken, Lindy Melegari, Gideon L Smith, Kris Grohn, Aaron J Wolfe, Kelsey Moody, Fadi Bou-Abdallah, Robert P Doyle. Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis. RSC medicinal chemistry. 2020 Sep 01;11(9):1048-1052

PMID: 33479697

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