Tubgcp3 is a mitotic regulator of planarian epidermal differentiation.

Cui Ge, Cun Lin, Mengzhen Zhang, Jingchen Yuan, Xiyan Feng, Zhitai Hao, Shoutao Zhang, Qingnan Tian

Gene 2021 Apr 05

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Tubgcp3/GCP3 (The centrosomal protein γ-tubulin complex protein 3) is a component of the γ-tubulin small complexes (γ-TuSCs) and γ-tubulin ring complexes (γ-TuRCs), which play critical roles in mitotic spindle formation during mitosis. However, its function in stem cell development has not been thoroughly elucidated. The planarian flatworm, which contains a large number of adult somatic stem cells (neoblasts), is a unique model to study stem cell lineage development in vivo. Here, we identified a homolog of Tubgcp3 in planarian Dugesia japonica, and found that Tubgcp3 is required for the maintenance of epidermal lineage. RNAi targeting Tubgcp3 resulted in tissue homeostasis and regeneration defect. Knockdown of Tubgcp3 reduced cell divisions and led to a loss of the mature epidermal cells. Our findings indicate that Tubgcp3 is a mitotic regulator and plays a crucial role in planarian epidermal differentiation. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Cui Ge, Cun Lin, Mengzhen Zhang, Jingchen Yuan, Xiyan Feng, Zhitai Hao, Shoutao Zhang, Qingnan Tian. Tubgcp3 is a mitotic regulator of planarian epidermal differentiation. Gene. 2021 Apr 05;775:145440