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Recently, functional polymers have attracted significant attention in the areas of pharmaceuticals and biomedical applications, so it is important to develop simple techniques to analyze functional polymers in their pharmaceutical dosage forms. Three simple, accurate, and sensitive UV spectrophotometric methods have been developed and validated for determination of polyvinyl pyrrolidone (PVP) in the presence of benzalkonium chloride (BZ) and sodium lactate in ternary mixtures. Method A is a derivative ratio spectra zero-crossing (DRSZ) method which measures PVP peak amplitude at 303.1 nm. Method B is a double divisor ratio derivative (DDRD), used for determination of both PVP and BZ in the presence of sodium lactate at 272.6 and 271.5 nm, respectively. Method C is a double divisor ratio derivative-ratio difference spectrophotometric method (DDRD-RDSM), a new and hybrid method of double divisor and ratio difference that hasn't been applied before. It measures peak amplitude difference of the ratio spectra at ΔP278 - 252.4 and ΔP260.9 - 213 for PVP and BZ, respectively. Linear ranges for PVP (5.00-35.00, 10.00-40.00, and 10.00-40.00 µg/mL) was obtained by using DRSZ, DDRD, and DDRD-RDSM, respectively. While the linear range for BZ (5.00-60 µg/mL) was obtained by using both DDRD and DDRD-RDSM. All results were statistically compared with reported methods. No significant differences were observed. The developed methods were applied to the analysis of the investigated drugs in pure and pharmaceutical dosage forms. The proposed methods are of great value, improving the efficiency of routine analysis of PVP and BZ in their pharmaceutical dosage forms. © AOAC INTERNATIONAL 2021. All rights reserved. For permissions, please email:


Nourhan Kh Al-Afify, Yossra A Trabik, Amira M El-Kosasy, N Magdy. Spectrophotometric Determination of Polyvinyl Pyrrolidone in Pure and Pharmaceutical Dosage Form. Journal of AOAC International. 2021 Sep 27;104(5):1420-1429

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PMID: 33484245

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