Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves.

The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method applying to the node of Ranvier of rat myelinated nerves. The step-by-step protocol described here allows researchers to apply this approach to study mechanisms underlying saltatory conduction and information processing in myelinated nerves of mammals. For complete information on the generation and use of this protocol, please refer to Kanda et al. (2019). © 2020 The Author(s).

Citation

Hirosato Kanda, Sotatsu Tonomura, Yi Dai, Jianguo G Gu. Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves. STAR protocols. 2021 Mar 19;2(1):100266

Mesh Tags

PMID: 33490982

search → result