Glucose absorption regulation and mechanism of the compounds in Lilium lancifolium Thunb on Caco-2 cells.

Xiaoqing Xu, Pengyu Wang, Baoguang Wang, Mengke Wang, Senye Wang, Zhenhua Liu, Yan Zhang, Wenyi Kang

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Mar

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In this paper, the Caco-2 cell was used to study the glucose absorption regulation and mechanism of kaempferol, caffeic acid and quercetin-3-O-β-D-galactoside in Lilium lancifolium Thunb in vitro. Glucose oxidase-peroxidase (GOD-POD) method was used to measure glucose consumption in supernatant. The fluorescent D-glucose analog (2-NBDG) was used as a tracer probe to study the changes in the fluorescence intensity of 2-NBDG uptake by Caco-2 cells with an inverted fluorescence microscope. Western blotting and quantitative real-time PCR were used to detect the protein expression and mRNA transcription of SGLT1 and GLUT2. The results showed that caffeic acid and quercetin-3-O-β-D-galactoside could significantly promote the absorption of glucose by normal Caco-2 cells compared with the control group (P < 0.001). Both caffeic acid and quercetin-3-O-β-D-galactoside could significantly promote the uptake of glucose tracer 2-NBDG on Caco-2 cells. Caffeic acid and quercetin-3-O-β-D-galactoside could significantly promote SGLT1 and GLUT2 protein expression levels and mRNA transcription (P < 0.001, P < 0.01, P < 0.05). The mechanism might be related to the promotion of SGLT1 and GLUT2 protein expression levels and mRNA transcription. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Xiaoqing Xu, Pengyu Wang, Baoguang Wang, Mengke Wang, Senye Wang, Zhenhua Liu, Yan Zhang, Wenyi Kang. Glucose absorption regulation and mechanism of the compounds in Lilium lancifolium Thunb on Caco-2 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2021 Mar;149:112010