Isolation of manumycin-type derivatives and genome characterization of a marine Streptomyces sp. C1-2.

Sakkarn Chanadech, Duangkamol Ruen-Ngam, Chakapong Intaraudom, Pattama Pittayakhajonwut, Sumet Chongruchiroj, Jaturong Pratuangdejkul, Chitti Thawai

Research in microbiology 2021 Mar

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A marine actinomycete strain C1-2 was taxonomically characterized as the genus Streptomyces, based on whole-genome sequence analysis. The highest average nucleotide identity (ANI) value (98.96%) and digital DNA-DNA hybridization (DDH) value (90.4%) were observed between Streptomyces sp. C1-2 and Streptomyces griseoaurantiacus. Thus, Streptomyces sp. C1-2 could be identified as S. griseoaurantiacus. Genome analysis revealed that Streptomyces sp. C1-2 contained 22 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 54% have low similarities with known BGCs. The chemical investigation led to the isolation of three new manumycin-type derivatives and two known analog antibiotics named SW-B and cornifronin B. All compounds showed antioxidant activity with the half-maximal inhibitory concentration (IC50) values in a range of 50.82 ± 0.8-112.04 ± 1.0 μg/mL with no cytotoxicity against Vero cells. This is the first report of the antioxidant property of manumycin-type derivatives. Moreover, two known compounds exhibited antifungal activity against Phytophthora capsici, Fusarium oxysporum f. sp. cucumerinum, and Magnaporthe grisea, with the minimum inhibitory concentration (MIC) values in a range of 125-500 μg/mL. Copyright © 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Citation

Sakkarn Chanadech, Duangkamol Ruen-Ngam, Chakapong Intaraudom, Pattama Pittayakhajonwut, Sumet Chongruchiroj, Jaturong Pratuangdejkul, Chitti Thawai. Isolation of manumycin-type derivatives and genome characterization of a marine Streptomyces sp. C1-2. Research in microbiology. 2021 Mar;172(2):103812