Smartphone based highly sensitive visualized detection of acid phosphatase enzyme activity.

Rui Li, Yanan Sun, Lihua Jin, Xiaohong Qiao, Cong Li, Yehua Shen

Analytical methods : advancing methods and applications 2021 Feb 14

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With the rapid development of point-of-care (POC) technologies, development of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for practical applications. In this study, we introduced a smartphone-based RGB analysis system for the sensitive detection of acid phosphatase (ACP) enzyme activity. In the presence of ACP, l-ascorbic acid 2-phosphate (AAP) can be converted into ascorbic acid (AA), which can reduce Ag+ to Ag0 and format the Au@Ag core-shell nanostructure. This morphology change of the Au@Ag core-shell would trigger a significant color variation (pink to yellow). A good linear relationship between the RGB model parameter and the concentration of ACP could be obtained with a detection limit of 0.1 U L-1. Moreover, this sensing strategy is suitable for the detection of ACP in practical serum samples. Thus, this simple but powerful protocol has great potential application for on-site detection of ACP in future complex biological samples.

Citation

Rui Li, Yanan Sun, Lihua Jin, Xiaohong Qiao, Cong Li, Yehua Shen. Smartphone based highly sensitive visualized detection of acid phosphatase enzyme activity. Analytical methods : advancing methods and applications. 2021 Feb 14;13(6):809-816