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    Herein, we established a novel deep eutectic solvent (DES) using lignin-derived guaiacol as hydrogen bond donor (HBD). The sole ChCl/guaiacol system was found to be inefficient for the fractionation of wheat straw (WS), while the incorporation of trace AlCl3 significantly facilitated the degradation of hemicellulose and lignin, resulting in a complete enzymatic digestibility of the pretreated WS. Further, this study revealed that the DES-degraded lignin was readily precipitated during the washing process, and thus hindered the enzymatic hydrolysis of poplar and bamboo (with hydrolysis yield of 42.03% and 71.67%, respectively). Alkali washing offers a possible approach to remove the precipitated lignin, after which a near 100% hydrolysis yield was also obtained for poplar and bamboo. Copyright © 2021 Elsevier Ltd. All rights reserved.


    Chen Huang, Yunni Zhan, Jinyuan Cheng, Jia Wang, Xianzhi Meng, Xuelian Zhou, Guigan Fang, Arthur J Ragauskas. Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment. Bioresource technology. 2021 Apr;326:124696

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    PMID: 33508646

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