Hydrolysis and transglycosylation activities of glycosidases from small intestine brush-border membrane vesicles.

Lesbia Cristina Julio-Gonzalez, F Javier Moreno, María Luisa Jimeno, Elisa G Doyagüez, Agustín Olano, Nieves Corzo, Oswaldo Hernandez-Hernandez

Food research international (Ottawa, Ont.) 2021 Jan

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In order to know the catalytic activities of the disaccharidases expressed in the mammalian small intestinal brush-border membrane vesicles (BBMV) high concentrated solutions of sucrose, maltose, isomaltulose, trehalose and the mixture sucrose:lactose were incubated with pig small intestine disaccharidases. The hydrolysis and transglycosylation reactions generated new di- and trisaccharides, characterized and quantified by GC-MS and NMR, except for trehalose where only hydrolysis was detected. In general, α-glucosyl-glucoses and α-glucosyl-fructoses were the most abundant structures, whereas no fructosyl-fructoses or fructosyl-glucoses were found. The in-depth structural characterization of the obtained carbohydrates represents a new alternative to understand the potential catalytic activities of pig small intestinal disaccharidases. The hypothesis that the oligosaccharides synthesized by glycoside hydrolases could be also hydrolysed by the same enzymes was confirmed. This information could be extremely useful in the design of new non-digestible or partially digestible oligosaccharides with potential prebiotic properties. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Lesbia Cristina Julio-Gonzalez, F Javier Moreno, María Luisa Jimeno, Elisa G Doyagüez, Agustín Olano, Nieves Corzo, Oswaldo Hernandez-Hernandez. Hydrolysis and transglycosylation activities of glycosidases from small intestine brush-border membrane vesicles. Food research international (Ottawa, Ont.). 2021 Jan;139:109940