Functional expression and localisation of HOPS/TMUB1 in mouse lens.

Daniela Bartoli, Danilo Piobbico, Marilena Castelli, Stefania Pieroni, Damiano Scopetti, Simona Ferracchiato, Nicola Di-Iacovo, Carlo Cagini, Giuseppe Servillo, Maria Agnese Della-Fazia

Bioscience reports 2021 Feb 26

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Transparency represents the functional phenotype of eye lens. A number of defined steps including quiescence, proliferation, migration and cell differentiation culminates in cell elongation and organelle degradation, allowing the light to reach the retina. HOPS (Hepatocyte Odd Protein Shuttling)/TMUB1 (Trans Membrane Ubiquitin-like containing protein 1) is a nucleo-cytoplasmic shuttling protein, highly expressed both in vivo and in vitro proliferating systems, bearing a ubiquitin-like domain. The present study shows HOPS expression during the phases of lens cell proliferation and fiber differentiation, and its localisation in lens compartments. In lens, HOPS localises mainly in the nucleus of central epithelial cells. During mitosis, HOPS/TMUB1 shuttles to the cytoplasm and returns to the nucleus at the end of mitosis. The differentiating cells share distinct HOPS/TMUB1 localisation in transitional zone depending on the differentiation phases. HOPS/TMUB1 is observed in lens cortex and nucleus. Here, it is attached to fibers, having a structural function with crystallin proteins, probably acting in the ubiquitin-proteasome system. © 2021 The Author(s).

Citation

Daniela Bartoli, Danilo Piobbico, Marilena Castelli, Stefania Pieroni, Damiano Scopetti, Simona Ferracchiato, Nicola Di-Iacovo, Carlo Cagini, Giuseppe Servillo, Maria Agnese Della-Fazia. Functional expression and localisation of HOPS/TMUB1 in mouse lens. Bioscience reports. 2021 Feb 26;41(2)