Distinct roles of LRP5 and LRP6 in Wnt signaling regulation in the retina.

Harminder D Singh, Jian-Xing Ma, Yusuke Takahashi

Biochemical and biophysical research communications 2021 Mar 19

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Dysregulation of Wnt signaling is implicated in multiple ocular disorders. The roles of Wnt co-receptors LRP5 and LRP6 in Wnt signaling regulation remain elusive, as most retinal cells express both of the co-receptors. To address this question, LRP5 and LRP6 were individually knocked-out in a human retinal pigment epithelium cell line using the CRISPR-Cas9 technology. Wnt signaling activity induced by various Wnt ligands was measured using wild-type and the KO cell lines. The results identified three groups of Wnt ligands based on their co-receptor specificity: 1) activation of Wnt signaling only through LRP6, 2) through both LRP5 and LRP6 and 3) predominantly through LRP5. These results indicate that LRP5 and LRP6 have differential roles in Wnt signaling regulation. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Harminder D Singh, Jian-Xing Ma, Yusuke Takahashi. Distinct roles of LRP5 and LRP6 in Wnt signaling regulation in the retina. Biochemical and biophysical research communications. 2021 Mar 19;545:8-13