Recombinant production of poly-(3-hydroxybutyrate) by Bacillus megaterium utilizing millet bran and rapeseed meal hydrolysates.

Subbi Rami Reddy Tadi, Som Dutt Ravindran, Rengesh Balakrishnan, Senthilkumar Sivaprakasam

Bioresource technology 2021 Apr

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Fermentative poly-3-hydroxybutyrate (PHB) production is mainly limited by the cost of raw material. In this present study, low-cost feedstock viz., millet bran residue (MBRH) and rapeseed meal hydrolysates were successfully utilized for PHB production. Metabolic engineering of Bacillus megaterium by co-expression of both precursor (phbRBC) and NADPH cofactor regeneration (zwf) genes resulted in 2.67-fold enhancement in PHB accumulation compared to wild strain. Modified logistic model characterized B.megaterium growth and PHB production effectively. The kinetic analysis proved that maximum cell concentration (15.01 g.L-1) and growth-associated constant (0.22 g.g-1) were found to be higher for initial MBRH concentration (S0 = 20 g.L-1). PHB production kinetics elucidated its expression in B.megaterium was growth-associated. PHB synthesized by B.megaterium was characterized using FTIR, NMR, XRD, DSC/TGA, FESEM and the physio-chemical properties enumerated its as a potential biodegradable plastic for industrial application. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Subbi Rami Reddy Tadi, Som Dutt Ravindran, Rengesh Balakrishnan, Senthilkumar Sivaprakasam. Recombinant production of poly-(3-hydroxybutyrate) by Bacillus megaterium utilizing millet bran and rapeseed meal hydrolysates. Bioresource technology. 2021 Apr;326:124800