Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the diversity of both antigen-specific and total B cells in healthy subjects and malaria-exposed individuals. This reveals two B cell lineages: a classical lineage of activated and resting memory B cells and an alternative lineage, which includes previously described atypical B cells. Although atypical B cells have previously been associated with disease states, the alternative lineage is common in healthy controls, as well as malaria-exposed individuals. We further track Plasmodium-specific B cells after malaria vaccination in naive volunteers. We find that alternative lineage cells are primed after the initial immunization and respond to booster doses. However, alternative lineage cells develop an atypical phenotype with repeated boosts. The data highlight that atypical cells are part of a wider alternative lineage of B cells that are a normal component of healthy immune responses. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.


Henry J Sutton, Racheal Aye, Azza H Idris, Rachel Vistein, Eunice Nduati, Oscar Kai, Jedida Mwacharo, Xi Li, Xin Gao, T Daniel Andrews, Marios Koutsakos, Thi H O Nguyen, Maxim Nekrasov, Peter Milburn, Auda Eltahla, Andrea A Berry, Natasha Kc, Sumana Chakravarty, B Kim Lee Sim, Adam K Wheatley, Stephen J Kent, Stephen L Hoffman, Kirsten E Lyke, Philip Bejon, Fabio Luciani, Katherine Kedzierska, Robert A Seder, Francis M Ndungu, Ian A Cockburn. Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans. Cell reports. 2021 Feb 09;34(6):108684

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 33567273

View Full Text