A colorimetric sensor for detecting thiourea based on inhibiting peroxidase-like activity of gold-platinum nanoparticles.

Yuqi Zhao, Qingjie Fu, Xiaoqian Cui, Hui Chi, Yongzhuang Lu, Xiaoli Liu, Miao Yu, Qiang Fei, Guodong Feng, Hongyan Shan, Yanfu Huan

Analytical methods : advancing methods and applications 2021 Feb 28

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In this study, gold-platinum nanoparticles (Au@PtNPs) with peroxidase-like activity were synthesized. In the absence of thiourea (TU), the Au@PtNPs can catalyze the decomposition of hydrogen peroxide, and oxidize 3,3',5,5'-tetramethylbenzidine dihydrochloride (TMB, colorless) into oxidized 3,3',5,5'-tetramethylbenzidine dihydrochloride (oxTMB, blue). The peroxidase-like activity of the Au@PtNPs is inhibited in the presence of TU, and TMB cannot be oxidized to oxTMB effectively, and no blue color could be observed. Based on this finding, a novel colorimetric sensor for detecting TU is proposed. The absorbance response curve showed a good linearity for the concentration of TU in the range of 10 nmol L-1 to 10 μmol L-1 with a correlation coefficient of R2 = 0.999, and the limit of detection is 9.57 nmol L-1. The colorimetric sensor possesses excellent selectivity, anti-interference ability, and application value in actual samples.

Citation

Yuqi Zhao, Qingjie Fu, Xiaoqian Cui, Hui Chi, Yongzhuang Lu, Xiaoli Liu, Miao Yu, Qiang Fei, Guodong Feng, Hongyan Shan, Yanfu Huan. A colorimetric sensor for detecting thiourea based on inhibiting peroxidase-like activity of gold-platinum nanoparticles. Analytical methods : advancing methods and applications. 2021 Feb 28;13(8):1069-1074