Antioxidant and antimicrobial material by grafting of L-arginine onto enzymatic poly(gallic acid).

Materials science & engineering. C, Materials for biological applications 2021 Feb

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Microwave-mediated grafting of L-Arg onto naturally derived and stable multiradical poly(gallic acid) (PGAL) in aqueous media has been successfully achieved. This polymeric material has no adverse effect in human cells as there is no hemolytic activity upon MTT and Neutral Red assays. The analytical and computational characterization studies carried out in this study describe a helical molecular structure with random incorporation of L-Arginine pendant groups from PGAL's backbone. The antioxidant properties of the precursor polymer are preserved as proved by the elimination of stable DPPH and hydroxyl radical scavenging, as well as the FRAP and ORAC assays. Regarding the latter, the oxygen radical inhibition is enhanced compared to PGAL, which is attributed to the guanidyl moieties. PGAL-g-L-Arg displays antimicrobial activity against Gram (+) Listeria monocytogenes and Staphylococcus aureus strains with a MIC of 0.8 g/L and a bacteriostatic effect against Gram (-) Escherichia coli. Additionally, scanning electron and confocal fluorescence microscopies as well as crystal violet colorimetric assay demonstrate that the mechanism involved in the bacterial inhibition is related to the formation of porous channels on the membrane, which is discussed according to the helical secondary structure of the polymer and the amino acid guanidyl moieties interacting to bacterial membranes. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Miguel A Hernández-Valdepeña, Carmen G Hernández-Valencia, Pablo Labra-Vázquez, Carmen Wacher, Gloria Díaz-Ruiz, Alfredo Vázquez, José Pedraza-Chaverri, Keiko Shirai, Alberto Rosas-Aburto, Eduardo Vivaldo-Lima, Eduardo Bárzana, Romina Rodríguez-Sonoja, Miquel Gimeno. Antioxidant and antimicrobial material by grafting of L-arginine onto enzymatic poly(gallic acid). Materials science & engineering. C, Materials for biological applications. 2021 Feb;121:111650