Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes.

Dhenesh Puvanendran, Hager Souabni, Dimitri Salvador, Olivier Lambert, Quentin Cece, Martin Picard

Methods in molecular biology (Clifton, N.J.) 2020

filter terms:

Proteoliposome reconstitution is a method of choice for the investigation of membrane proteins as it allows their manipulation in the desired hydrophobic environment and allows one to tackle their study from both functional and structural points of view. Methods for their rapid and efficient reconstitution have been known for a long time but the quality and dispersity of the resulting suspensions is often overlooked. Here we describe our routine for the obtention of monodisperse populations of proteoliposomes as well as for the quantitation of protein per liposome.

Citation

Dhenesh Puvanendran, Hager Souabni, Dimitri Salvador, Olivier Lambert, Quentin Cece, Martin Picard. Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes. Methods in molecular biology (Clifton, N.J.). 2020;2168:63-72