Co-infections of Plasmodium relictum lineages pSGS1 and pGRW04 are readily distinguishable by broadly used PCR-based protocols, with remarks on global distribution of these malaria parasites.

Plasmodium relictum is the most common generalist avian malaria parasite, which was reported in over 300 bird species of different orders, particularly often in passerines. This malaria infection is often severe in non-accustomed avian hosts. Currently, five distinct cytochrome b gene lineages have been assigned to P. relictum, with the lineages pSGS1 and pGRW04 being the most common. Based on molecular screenings, the transmission of these two parasite lineages might occur in sympatry, particularly often in sub-Saharan Africa, but they also have been reported to have different areas of transmission globally, with the lineages pSGS1 and pGRW04 being of low (if at all) transmission in huge regions of Americas and Europe, respectively. It remains unclear why these lineages are more often reported in some geographical areas, even though their susceptible vertebrate hosts and vectors are present globally. Co-infections of malaria parasites and other haemosporidians belonging to different species and subgenera are prevalent and even predominate in many bird populations, however, PCR-based protocols using commonly used primers often do not read such co-infections. Because information about the sensitivity of these protocols to read co-infections of the lineages pSGS1 and pGRW04 is absent, this study aimed to unravel this issue experimentally. Blood samples of birds experimentally infected with the single parasite lineages pSGS1 and pGRW04 were used to prepare various combinations of mixes, which were tested by two PCR-based protocols, which have been often used in current avian malaria research. Single infections of the same lineages were used as controls. Careful examination of the sequence electropherograms showed the presence of clear double peaks on polymorphic sites, indicating co-infections. This experiment shows that the broadly used PCR-based protocols can readily distinguish co-infections of these parasite lineages. In other words, the available information about patterns of the geographical distribution of the P. relictum lineages pSGS1 and pGRW04 likely mirrors the existing epidemiological situation but is not a result of the bias due to preferable DNA amplification of one of these lineages during their possible co-infections. This calls for further ecological research aiming determination of factors associated with the transmission of the lineages pSGS1 and pGRW04 in different regions of the globe. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Carolina Romeiro Fernandes Chagas, Josef Harl, Gediminas Valkiūnas. Co-infections of Plasmodium relictum lineages pSGS1 and pGRW04 are readily distinguishable by broadly used PCR-based protocols, with remarks on global distribution of these malaria parasites. Acta tropica. 2021 May;217:105860

Mesh Tags

Substances

PMID: 33587942

search → result