Investigating the microbial dynamics of microcystin-LR degradation in Lake Erie sand.

Chelsea Salter, Danielle VanMensel, Thomas Reid, Johnna Birbeck, Judy Westrick, Scott O C Mundle, Christopher G Weisener

Chemosphere 2021 Jun

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Cyanobacterial blooms and the associated hepatotoxins produced (e.g., microcystins, MCs) create a significant human health risk in freshwater lakes around the world, including Lake Erie. Though various physical and chemical treatment options are utilized, these are costly and their effectiveness decreases when other organics are present. Laboratory studies have identified a remediation option based on a mlr gene operon that can systematically degrade this toxin; however, studies on Lake Erie have been unable to amplify mlr genes from MC-degrading bacteria. These results suggest that either existing primers may be inefficient for broad identification of the mlr genes or that MC degradation genes and/or pathways may vary among bacterial taxa. To investigate the dynamics of the Lake Erie microbial community involved in the degradation of microcystin-LR (MC-LR), a flow-through column experiment using collected beach sand was conducted over a period of six weeks. Increasing concentrations of lake water spiked with MC-LR were continuously delivered to both biotic and abiotic (sterilized) sand columns, with influent and effluent MC-LR concentrations measured by LC-MS/MS. Despite the toxin concentrations far exceeding natural conditions during a bloom event (maximum dosage = 15.4 μg/L), MC-LR was completely removed within 21 h of contact time in the biotic columns. Stimulation of community taxa during the degradation process included Burkholderiaceae, Illumatobacteraceae, Pseudomonadaceae, Rhodocyclaceae and Nitrosomonadaceae. The overall results suggest several critical species may be required for the most complete and effective degradation of MC-LR. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Chelsea Salter, Danielle VanMensel, Thomas Reid, Johnna Birbeck, Judy Westrick, Scott O C Mundle, Christopher G Weisener. Investigating the microbial dynamics of microcystin-LR degradation in Lake Erie sand. Chemosphere. 2021 Jun;272:129873