A graphene-based fluorescent nanoprobe for simultaneous imaging of dual miRNAs in living cells.

Xiang Xiong, Wenya Dang, Ruxin Luo, Ying Long, Chunyi Tong, Liqin Yuan, Bin Liu

Talanta 2021 Apr 01

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MicroRNAs (miRNAs) are regarded as important biomarkers for disease diagnostics and therapeutics due to their significant regulatory roles in physiologic and pathologic processes. Herein, a versatile nanoprobe based on reduced graphene oxide (rGO) and nucleic acid (DNA) probe was prepared for simultaneously visualize miR-451a and miR-214-3p in living cells. In vitro experiments demonstrated that the nanoprobe exhibits excellent selectivity and outstanding sensitivity as low as 1 nM towards miR-451a and miR-214-3p. Moreover, the detection signals of miRNAs have good linearity in their respective concentration ranges (miR-451a: 1-100 nM, Y1 = 9.3062X1+114.85 (R2 = 0.9965). miR-214-3p: 1-200 nM, Y2 = 1.4424X2+91.312 (R2 = 0.9961)). Finally, simultaneous dual-color imaging of miR-451a and miR-214-3p in human breast cancer cells (MDA-MB-231) was realized by exploiting the P1&P2@rGO nanoprobe. In summary, this simple and effective strategy provides a general sensing platform for highly sensitive detection and simultaneous imaging of dual miRNAs in living cells. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Xiang Xiong, Wenya Dang, Ruxin Luo, Ying Long, Chunyi Tong, Liqin Yuan, Bin Liu. A graphene-based fluorescent nanoprobe for simultaneous imaging of dual miRNAs in living cells. Talanta. 2021 Apr 01;225:121947