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Multiregion sequencing can advance our understanding of the intratumor heterogeneity and the clonal evolution. Here, we introduced multiple aspects of multiregion sequencing and its analysis, including the study design and sampling strategy, current understanding of the tumor evolution model, and a protocol for multiregion sequencing analysis of DNA-sequencing data.


Soyeon Ahn, Haiyan Huang. Multiregion Sequence Analysis to Predict Intratumor Heterogeneity and Clonal Evolution. Methods in molecular biology (Clifton, N.J.). 2021;2243:283-296

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PMID: 33606263

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