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    Neurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we report the generation of two iPS cell lines, where DOX inducible constructs of neurog2 either without or with T2A-eGFP were inserted into the safe-site locus AAVS1. These iPS cell lines, BIONi010-C-13 and BIONi010-C-15, respectively, stay pluripotent without DOX but differentiate to (GFP positive) neurons when DOX is added without the need of differentiation factors. Copyright © 2021 Janssen Pharmaceutica. Published by Elsevier B.V. All rights reserved.

    Citation

    Benjamin Schmid, Bjørn Holst, Ulla Poulsen, Ida Jørring, Christian Clausen, M Rasmussen, Ulrike A Mau-Holzmann, Rachel Steeg, Hugh Nuthall, A Ebneth, A Cabrera-Socorro. Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus. Stem cell research. 2021 Apr;52:102240

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    PMID: 33610017

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