Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus.

Neurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we report the generation of two iPS cell lines, where DOX inducible constructs of neurog2 either without or with T2A-eGFP were inserted into the safe-site locus AAVS1. These iPS cell lines, BIONi010-C-13 and BIONi010-C-15, respectively, stay pluripotent without DOX but differentiate to (GFP positive) neurons when DOX is added without the need of differentiation factors. Copyright © 2021 Janssen Pharmaceutica. Published by Elsevier B.V. All rights reserved.

Citation

Benjamin Schmid, Bjørn Holst, Ulla Poulsen, Ida Jørring, Christian Clausen, M Rasmussen, Ulrike A Mau-Holzmann, Rachel Steeg, Hugh Nuthall, A Ebneth, A Cabrera-Socorro. Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus. Stem cell research. 2021 Apr;52:102240

Mesh Tags

PMID: 33610017

search → result