Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation.

Stacey M Thomas, Joseph Bednarek, William J Janssen, Patrick S Hume

Journal of visualized experiments : JoVE 2021 Feb 02

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Lung histology is often used to investigate the contributions provided by airspace cells during lung homeostasis and disease pathogenesis. However, commonly used instillation-based fixation methods can displace airspace cells and mucus into terminal airways and can alter tissue morphology. In comparison, vascular perfusion-fixation techniques are superior at preserving the location and morphology of cells within airspaces and the mucosal lining. However, if positive airway pressure is not simultaneously applied, regions of the lungs may collapse and capillaries may bulge into the alveolar spaces, leading to distortion of the lung anatomy. Herein, we describe an inexpensive method for air-inflation during vascular perfusion-fixation to preserve the morphology and location of airway and alveolar cells and interstitium in murine lungs for downstream histologic studies. Constant air pressure is delivered to the lungs via the trachea from a sealed, air-filled chamber that maintains pressure via an adjustable liquid column while fixative is perfused through the right ventricle.

Citation

Stacey M Thomas, Joseph Bednarek, William J Janssen, Patrick S Hume. Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation. Journal of visualized experiments : JoVE. 2021 Feb 02(168)