Conserved role of ATP synthase in mammalian cilia.

Wenjun Lin, Cheng Qiao, Jinghua Hu, Qing Wei, Tao Xu

Experimental cell research 2021 Apr 01

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We previously found that ATP synthases localize to male-specific sensory cilia and control the ciliary response by regulating polycystin signalling in Caenorhabditis elegans. Herein, we discovered that the ciliary localization of ATP synthase is evolutionarily conserved in mammals. We showed that the ATP synthase subunit F1β is colocalized with the cilia marker acetylated α-tubulin in both mammalian renal epithelial cells (MDCK) and normal mouse cholangiocytes (NMCs). Treatment with ATP synthase inhibitor oligomycin impaired ciliogenesis in MDCK cells, and F1β was co-immunoprecipitated with PKD2 in mammalian cells. Our study provides evidence for the evolutionarily conserved localization of ATP synthase in cilia from worm to mammals. Defects in ATP synthase can lead to ciliary dysfunction, which may be a potential mechanism of polycystic kidney disease. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Wenjun Lin, Cheng Qiao, Jinghua Hu, Qing Wei, Tao Xu. Conserved role of ATP synthase in mammalian cilia. Experimental cell research. 2021 Apr 01;401(1):112520