In vitro (poly)phenol catabolism of unformulated- and phytosome-formulated cranberry (Vaccinium macrocarpon) extracts.

Letizia Bresciani, Giuseppe Di Pede, Claudia Favari, Luca Calani, Veronica Francinelli, Antonella Riva, Giovanna Petrangolini, Pietro Allegrini, Pedro Mena, Daniele Del Rio

Food research international (Ottawa, Ont.) 2021 Mar

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Cranberries (Vaccinium macrocarpon) represent an important source of anthocyanins, flavan-3-ols and flavonols. This study aimed at investigating in vitro the human microbial metabolism of (poly)phenols, principally flavan-3-ols, of unformulated- and phytosome-formulated cranberry extracts. After powder characterization, a 24-h fermentation with human faecal slurries was performed, standardizing the concentration of incubated proanthocyanidins. Cranberry (poly)phenol metabolites were quantified by uHPLC-MS2 analyses. The native compounds of both unformulated- and phytosome-formulated cranberry extracts were metabolized under faecal microbiota activity, resulting in twenty-four microbial metabolites. Although some differences appeared when considering different classes of colonic metabolites, no significant differences in the total amount of metabolites were established after 24 h of incubation period. These results suggested that a different formulation had no effect on flavan-3-ol colonic metabolism of cranberry and both unformulated- and phytosome-formulated extract. Both formulations displayed the capability to be a potential source of compounds which could lead to a wide array of gut microbiota metabolites in vitro. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Letizia Bresciani, Giuseppe Di Pede, Claudia Favari, Luca Calani, Veronica Francinelli, Antonella Riva, Giovanna Petrangolini, Pietro Allegrini, Pedro Mena, Daniele Del Rio. In vitro (poly)phenol catabolism of unformulated- and phytosome-formulated cranberry (Vaccinium macrocarpon) extracts. Food research international (Ottawa, Ont.). 2021 Mar;141:110137