Methodological study on eliminating nucleic acid contamination in molecular diagnostic laboratory].

Yunlong Li, Jian Zhang, Yanqiu Wei, Xiaojuan Jia, Xiaoyan Li, Liming Tan, Wenjun Liu, Limin Yang

Sheng wu gong cheng xue bao = Chinese journal of biotechnology 2021 Feb 25

filter terms:

Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.

Citation

Yunlong Li, Jian Zhang, Yanqiu Wei, Xiaojuan Jia, Xiaoyan Li, Liming Tan, Wenjun Liu, Limin Yang. Methodological study on eliminating nucleic acid contamination in molecular diagnostic laboratory]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 2021 Feb 25;37(2):673-679