Analysis of Copy Number Variation in Urine: c-Myc Evaluation Using a Real-Time PCR Approach.

Valentina Casadio, Filippo Martignano, Roberta Gunelli, Samanta Salvi

Methods in molecular biology (Clifton, N.J.) 2021

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Urine cell-free DNA has been shown as an informative noninvasive source of biomarkers for a number of diseases, especially for urological cancers. Starting from the hypothesis that the gain of c-Myc gene is a frequent aberration in several cancer types, including prostate cancer, we analyzed c-Myc copy number variation in urine, studying a little case series of prostate cancer patients, to test its feasibility. Here we report a general protocol that may be considered to analyze gene copy number variation in the urine cell-free fraction.

Citation

Valentina Casadio, Filippo Martignano, Roberta Gunelli, Samanta Salvi. Analysis of Copy Number Variation in Urine: c-Myc Evaluation Using a Real-Time PCR Approach. Methods in molecular biology (Clifton, N.J.). 2021;2292:49-56