Expression of Luteinizing Hormone-Releasing Hormone (LHRH) and Type-I LHRH Receptor in Transitional Cell Carcinoma Type of Human Bladder Cancer.

Zsuzsanna Szabó, Balázs Dezső, Klára Fodor, Krisztián Szegedi, Tibor Flaskó, Erzsébet Szabó, Gábor Oláh, Éva Sipos, Nikoletta Dobos, János Gardi, Andrew V Schally, Gábor Halmos

Molecules (Basel, Switzerland) 2021 Feb 26

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Bladder cancer (BC) is the tenth most frequently detected cancer in both sexes. Type-I luteinizing hormone-releasing hormone (LHRH) receptor (LHRH-R-I) is expressed not only in the pituitary, but also in several types of cancer disease. There are few data about LHRH-R-I expression in human BC. This study aimed to investigate the expression of LHRH and LHRH-R-I in the transitional cell carcinoma (TCC) type of human BC. RNA was extracted from 24 human bladder tumor specimens and three BC cell lines. RT-PCR was performed to detect mRNA for LHRH and LHRH-R-I. The protein of LHRH-R-I was further studied by immunohistochemistry (IHC), ligand competition assay, and Western Blot. PCR products of LHRH were found in 19 of 24 (79%) specimens and mRNA of LHRH-R-I was detected in 20 of 24 specimens (83%). Positive immunostaining for LHRH-R-I with different expression intensity was found in all samples examined, showing negative correlation with TCC grade. Radioligand binding studies also showed the presence of specific LHRH-R-I and high affinity binding of LHRH analogs. The high incidence of LHRH-R in BC suggests that it could serve as a molecular target for therapy of human BC with cytotoxic LHRH analogs or modern powerful antagonistic analogs of LHRH.

Citation

Zsuzsanna Szabó, Balázs Dezső, Klára Fodor, Krisztián Szegedi, Tibor Flaskó, Erzsébet Szabó, Gábor Oláh, Éva Sipos, Nikoletta Dobos, János Gardi, Andrew V Schally, Gábor Halmos. Expression of Luteinizing Hormone-Releasing Hormone (LHRH) and Type-I LHRH Receptor in Transitional Cell Carcinoma Type of Human Bladder Cancer. Molecules (Basel, Switzerland). 2021 Feb 26;26(5)