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Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay.
Xiaolong Hao, Ling Li
Methods in molecular biology (Clifton, N.J.) 2021
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The LexA-based yeast two-hybrid system is one of the most powerful techniques used to detect blue light-dependent protein-protein interactions. In Arabidopsis, many protein-protein interactions in blue light signaling pathway were identified using this system. Here we present an easy and efficient method of the LexA-based yeast two-hybrid assay for testing protein-protein interactions in a blue light-dependent manner.
Citation
Xiaolong Hao, Ling Li.
Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay.
Methods in molecular biology (Clifton, N.J.).
2021;2297:147-154
Mesh Tags
Arabidopsis
Arabidopsis Proteins
Bacterial Proteins
Color
Genetic Vectors
Leucine
Light
Plasmids
Protein Interaction Mapping
Saccharomyces cerevisiae
Serine Endopeptidases
Signal Transduction
Transformation, Genetic
Two-Hybrid System Techniques
beta-Galactosidase
Substances
Arabidopsis Proteins
Bacterial Proteins
LexA protein, Bacteria
beta-Galactosidase
Serine Endopeptidases
Leucine
PMID: 33656678
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