Protective effect of baicalein on DNA oxidative damage and its binding mechanism with DNA: An in vitro and molecular docking study.

Rui Wang, Jian Li, De-Bao Niu, Fei-Yue Xu, Xin-An Zeng

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 May 15

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In this work, the protective effect of baicalein on DNA oxidative damage and its possible protection mechanisms were investigated. 2-thiobarbituric acid (TBA) colorimetry and agarose gel electrophoresis study found that baicalein protected the deoxyribose residue and double-stranded backbone of DNA from the damage of hydroxyl radicals. Antioxidant analysis results showed that baicalein has excellent radicals scavenging effects and Fe2+ chelating ability, which might be the mechanism of baicalein protecting DNA. DNA binding studies indicated that baicalein bound to the minor groove of DNA with moderate binding affinity (K = (7.35 ± 0.91) × 103 M-1). Hydrogen bonding and van der Waals forces played a major role in driving the binding process. Molecular docking further confirmed the experimental results. This binding could stabilize DNA double helix structure, thereby protecting DNA from oxidative damage. This study may provide theoretical basis for designing new functional foods of baicalein for DNA damage protection. Copyright © 2021. Published by Elsevier B.V.

Citation

Rui Wang, Jian Li, De-Bao Niu, Fei-Yue Xu, Xin-An Zeng. Protective effect of baicalein on DNA oxidative damage and its binding mechanism with DNA: An in vitro and molecular docking study. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2021 May 15;253:119605