How to Select Firefly Luciferin Analogues for In Vivo Imaging.

Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

Citation

Ryohei Saito-Moriya, Jun Nakayama, Genta Kamiya, Nobuo Kitada, Rika Obata, Shojiro A Maki, Hiroshi Aoyama. How to Select Firefly Luciferin Analogues for In Vivo Imaging. International journal of molecular sciences. 2021 Feb 12;22(4)

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PMID: 33673331

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