Golgi apparatus components in Entamoeba histolytica and Entamoeba dispar after monensin treatment.

Daniel Talamás-Lara, Karla Acosta-Virgen, Bibiana Chávez-Munguía, Anel Lagunes-Guillén, Lizbeth Salazar-Villatoro, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo

Microscopy research and technique 2021 Aug

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Highly dynamic ribosomes, glycogen granules, thinly fibrillar material, and multiple membrane-bound vesicles are embedded in the matrix-rich cytoplasm of Entamoeba spp. trophozoites. The absence of a Golgi apparatus in these amoebae has been commonly accepted. Here we challenge this observation by incubating Entamoeba histolytica and Entamoeba dispar with monensin, an ionophore that produces swelling of the Golgi apparatus. We observe changes in the trophozoites through standard transmission electron microscopy, cryofixation and cryosubstitution, and analyze the label and expression of known resident proteins of the cis-GM130 and trans-TGN38 Golgi network through confocal microscopy and Western blot assays. Cryosubstitution and standard methods using the treatment, preserved membranous lamellae resembling Golgi components. GM130 and TGN38 Golgi antigens were found by immunoelectron, immunoblot, and co-localization by confocal microscopy using the reagent NBD C6-ceramide. Our results indicate that previously undetected Golgi apparatus components are present in the cytoplasm of E. histolytica and E. dispar. © 2021 Wiley Periodicals LLC.

Citation

Daniel Talamás-Lara, Karla Acosta-Virgen, Bibiana Chávez-Munguía, Anel Lagunes-Guillén, Lizbeth Salazar-Villatoro, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo. Golgi apparatus components in Entamoeba histolytica and Entamoeba dispar after monensin treatment. Microscopy research and technique. 2021 Aug;84(8):1887-1896