Correlation Engine 2.0
Clear Search sequence regions


  • adducts (2)
  • aldehydes (2)
  • dna adducts (4)
  • dna damage (1)
  • lipid (1)
  • lung (1)
  • mass (3)
  • rat (2)
  • tobacco smoke (1)
  • Sizes of these terms reflect their relevance to your search.

    Aldehydes are abundantly present in tobacco smoke and in urban air pollution and are endogenously generated as products of the lipid peroxidation process. These molecules can react with DNA bases forming mutagenic exocyclic adducts, which have been used as biomarkers of aldehyde exposure and as potential tools for the study of inflammation, metal storage diseases, neurodegenerative disorders, and cancer. High-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) provides a highly precise, specific and ultrasensitive method for the detection of exocyclic DNA adducts. Here we present and describe a validated micro-HPLC-Electro Spray Ionization (ESI)-MS/MS method for the quantification of 1,N2-propanodGuo, an adduct produced following the reaction between 2'-deoxyguanosine and acetaldehyde or crotonaldehyde.

    Citation

    Angélica B Sanchez, Camila C M Garcia, Paolo Di Mascio, Marisa H G Medeiros. Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach. Methods in molecular biology (Clifton, N.J.). 2021;2279:225-239

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 33683698

    View Full Text