Spatially Selective Imaging of Cell-Matrix and Cell-Cell Junctions by Electrochemiluminescence.

Hao Ding, Ping Zhou, Wenxuan Fu, Lurong Ding, Weiliang Guo, Bin Su

Angewandte Chemie (International ed. in English) 2021 May 17

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Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of cell junctions by electrochemiluminescence (ECL) microscopy. By regulating the concentrations of luminophore and/or co-reactant, the thickness of ECL layer can be controlled to match with the spatial location of different cell junctions. At a low concentration of luminophore, ECL generation is confined to the electrode surface, thus revealing only cell-matrix adhesions at the bottom of cells. While at a high concentration of luminophore, the ECL layer can be remarkably extended by decreasing the co-reactant concentration, thus allowing the sequential imaging of cell-matrix and cell-cell junctions at the bottom and near the apical surface of cells, respectively. This strategy not only provides new insights into the ECL mechanisms but also promises wide applications of ECL microscopy in bioimaging. © 2021 Wiley-VCH GmbH.

Citation

Hao Ding, Ping Zhou, Wenxuan Fu, Lurong Ding, Weiliang Guo, Bin Su. Spatially Selective Imaging of Cell-Matrix and Cell-Cell Junctions by Electrochemiluminescence. Angewandte Chemie (International ed. in English). 2021 May 17;60(21):11769-11773