PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization.

Kenichi Matsuda, Kei Fujita, Toshiyuki Wakimoto

Journal of industrial microbiology & biotechnology 2021 Jun 04

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Penicillin-binding protein-type thioesterases (PBP-type TEs) are a recently identified group of peptide cyclases that catalyze head-to-tail macrolactamization of nonribosomal peptides. PenA, a new member of this group, is involved in the biosyntheses of cyclic pentapeptides. In this study, we demonstrated the enzymatic activity of PenA in vitro, and analyzed its substrate scope with a series of synthetic substrates. A comparison of the reaction profiles between PenA and SurE, a representative PBP-type TE, showed that PenA is more specialized for small peptide cyclization. A computational model provided a possible structural rationale for the altered specificity for substrate chain lengths. © The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.

Citation

Kenichi Matsuda, Kei Fujita, Toshiyuki Wakimoto. PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization. Journal of industrial microbiology & biotechnology. 2021 Jun 04;48(3-4)