Designing enzyme cocktails from Penicillium and Aspergillus species for the enhanced saccharification of agro-industrial wastes.

Bioresource technology 2021 Jun

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The aim of this study was to develop optimized enzyme cocktails, containing native and recombinant purified enzymes from five fungal species, for the saccharification of alkali- and acid-pretreated sugarcane bagasse (SCB), soybean hulls (SBH) and oil palm empty fruit bunches (EFB). Basic cellulases were represented by cellobiohydrolase I (CBH) and endo-glucanase II (EG) from Penicillium verruculosum and β-glucosidase (BG) from Aspergillus niger. Auxiliary enzymes were represented by endo-xylanase A (Xyl), pectin lyase (PNL) and arabinoxylanhydrolase (AXH) from Penicillium canescens, β-xylosidase (BX) from Aspergillus japonicus, endo-arabinase (ABN) from A. niger and arabinofuranosidase (Abf) from Aspergillus foetidus. Enzyme loads were 5 mg protein/g dry substrate (basic cellulases) and 1 mg/g (each auxiliary enzyme). The best choice for SCB and EFB saccharification was alkaline pretreatment and addition of Xyl + BX, AXH + BX or ABN + BX + Abf to basic cellulases. For SBH, acid pretreatment and basic cellulases combined with ABN + BX + Abf or Xyl + BX performed better than other enzyme preparations. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Susan G Karp, Alexandra M Rozhkova, Margarita V Semenova, Dmitrii O Osipov, Suellen T Z de Pauli, Olga A Sinitsyna, Ivan N Zorov, Luciana Porto de Souza Vandenberghe, Carlos R Soccol, Arkady P Sinitsyn. Designing enzyme cocktails from Penicillium and Aspergillus species for the enhanced saccharification of agro-industrial wastes. Bioresource technology. 2021 Jun;330:124888