Correlation Engine 2.0
Clear Search sequence regions

  • cellular (1)
  • chemotaxis (4)
  • linear model (1)
  • movement (1)
  • partial (2)
  • t cells (1)
  • Sizes of these terms reflect their relevance to your search.

    Swarming has been observed in various biological systems from collective animal movements to immune cells. In the cellular context, swarming is driven by the secretion of chemotactic factors. Despite the critical role of chemotactic swarming, few methods to robustly identify and quantify this phenomenon exist. Here, we present a novel method for the analysis of time series of positional data generated from realizations of agent-based processes. We convert the positional data for each individual time point to a function measuring agent aggregation around a given area of interest, hence generating a functional time series. The functional time series, and a more easily visualized swarming metric of agent aggregation derived from these functions, provide useful information regarding the evolution of the underlying process over time. We extend our method to build upon the modelling of collective motility using drift-diffusion partial differential equations (PDEs). Using a functional linear model, we are able to use the functional time series to estimate the drift and diffusivity terms associated with the underlying PDE. By producing an accurate estimate for the drift coefficient, we can infer the strength and range of attraction or repulsion exerted on agents, as in chemotaxis. Our approach relies solely on using agent positional data. The spatial distribution of diffusing chemokines is not required, nor do individual agents need to be tracked over time. We demonstrate our approach using random walk simulations of chemotaxis and experiments investigating cytotoxic T cells interacting with tumouroids.


    Jack D Hywood, Gregory Rice, Sophie V Pageon, Mark N Read, Maté Biro. Detection and characterization of chemotaxis without cell tracking. Journal of the Royal Society, Interface. 2021 Mar;18(176):20200879

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 33715400

    View Full Text