Expressions of CD44, PCNA and MRP1 in lung cancer tissues and their effects on proliferation and invasion abilities of lung cancer cell line 95D.

Xudong Chen, Jinlin Sun, Yansen Wang

Journal of B.U.ON. : official journal of the Balkan Union of Oncology 2021 Jan-Feb

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To investigate the expressions of CD44 non-small cell lung cancer cells, proliferating cell nuclear antigen (PCNA) and multidrug resistance-associated protein 1 (MRP1) in the lung cancer tissues and their effects on the proliferation and invasion abilities in vitro of lung cancer cell line 95D. 138 lung cancer tissues and 127 adjacent normal tissues were collected from lung cancer patients after operation in Shandong Provincial Third Hospital from January 2015 to December 2017. CD44 siRNA (experimental CD44 group), PCNA siRNA (experimental PCNA group) and MRP1 siRNA (experimental MRP1 group) were transfected into human lung cancer 95D cells, and a negative control group (cells transfected with miR-Native Control) and a blank group (untransfected cells) were established. MTT assay was used for detecting the proliferation of cells, and Transwell chamber was used for detecting their invasion ability. The relative expressions of CD44, PCNA and MRP1 in the lung cancer tissues were higher than those in the adjacent tissues (p<0.050). At 24th h, the cell survival rate in the experimental MRP1 group was lower than that in the experimental PCNA group (p<0.050); At 48th the cell survival rate in the experimental MRP1 group was higher than that in the experimental CD44 group (p<0.050). At 72th h, the cell survival rate in the experimental PCNA group was significantly higher than that in the experimental CD44 group and the experimental MRP1 group (p<0.05). The cell invasion number in the experimental CD44 group, the experimental PCNA group and the experimental MRP1 group were significantly lower than cells in the negative control group and blank group (p<0.05). CD44, PCNA and MRP1, which may be involved in the regulation of the proliferation and invasion abilities of lung cancer cells, may serve as new molecular targeting markers for the diagnosis and treatment of lung cancer.

Citation

Xudong Chen, Jinlin Sun, Yansen Wang. Expressions of CD44, PCNA and MRP1 in lung cancer tissues and their effects on proliferation and invasion abilities of lung cancer cell line 95D. Journal of B.U.ON. : official journal of the Balkan Union of Oncology. 2021 Jan-Feb;26(1):72-78