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    Mast cell-deficient mice are helpful for understanding the roles of mast cells in vivo. To date, a dozen mouse models for mast cell deficiency have been reported. However, mice with a specific depletion of all populations of mast cells have not been reported. We generated knock-in mice, termed Mcpt5/Cma1DTR mice, expressing human diphtheria toxin A (DT) receptor under the endogenous promoter of Mcpt5 (also known as Cma1), which encodes mouse mast cell protease-5. Flow cytometry and histological analysis showed that intraperitoneal injection of DT induced almost complete depletion of mast cells in heterozygote Mcpt5/Cma1DTR/+ mice. The deletion rates of mast cells in peritoneal cavity, mesentery, abdominal skin, ear skin, and glandular stomach were 99.9%, 100%, 98.7%, 97.7%, and 100%, respectively. Passive cutaneous anaphylaxis reaction also revealed mast cell deficiency in ear skin after DT treatment. Other than mast cells, a small percentage of marginal zone B cells in Mcpt5/Cma1DTR/+ mice were killed by DT treatment. In conclusion, the Mcpt5/Cma1DTR/+ mouse model is valuable for achieving conditional depletion of all populations of mast cells without inducing a marked reduction in other cells. Copyright © 2021 Elsevier Inc. All rights reserved.


    Hayato Sasaki, Madoka Imanishi, Daisuke Fujikura, Makoto Sugiyama, Kyosuke Tanimoto, Yohei Mochiji, Yuki Takahashi, Koki Hiura, Masaki Watanabe, Takashige Kashimoto, Kenta Nakano, Tadashi Okamura, Nobuya Sasaki. New inducible mast cell-deficient mouse model (Mcpt5/Cma1DTR). Biochemical and biophysical research communications. 2021 Apr 30;551:127-132

    PMID: 33725574

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