Correlation Engine 2.0
Clear Search sequence regions

  • blood sugar (1)
  • enzymes (1)
  • glycogen (10)
  • liver (1)
  • liver glycogen (4)
  • mice (1)
  • organ (1)
  • particles (1)
  • polymer (1)
  • Sizes of these terms reflect their relevance to your search.

    Liver glycogen is a branched glucose polymer that functions as a blood-sugar buffer in animals. Previous studies have shown that glycogen's molecular structure affects its properties. This makes it important to develop a technique that extracts and purifies a representative sample of glycogen. Here we aim to optimize the sucrose density gradient centrifugation method for preserving glycogen's molecular structure by varying the density of the sucrose solution. The preservation of glycogen's structure involves: 1) minimizing molecular damage and 2) obtaining a structurally representative sample of glycogen. The addition of a 10-minute boiling step was also tested as a means for denaturing any glycogen degrading enzymes. Lower sucrose concentrations and the introduction of the boiling step were shown to be beneficial in obtaining a more structurally representative sample, with the preservation of smaller glycogen particles and decreased glycogen chain degradation. Crown Copyright © 2021. Published by Elsevier Ltd. All rights reserved.


    Ziyi Wang, Qinghua Liu, Liang Wang, Robert G Gilbert, Mitchell A Sullivan. Optimization of liver glycogen extraction when considering the fine molecular structure. Carbohydrate polymers. 2021 Jun 01;261:117887

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 33766374

    View Full Text