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    Non-human primate induced pluripotent cells (iPS cells) are useful for preclinical studies of iPS cell-based therapies and the research of primate developments. Since the initial report of iPS cells in 2006, various iPS cell induction methods have been reported. Here, we describe an efficient method for inducing iPS cells using a combination of RNA transfection and chemical compounds without using transgenes. Many kinds of marmoset cells, including difficult-to-reprogram cells, can be converted into iPS cells using this combinatorial method. Furthermore, this method can be applied to other primates, including humans.

    Citation

    Toshiaki Watanabe, Erika Sasaki. Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds. Methods in molecular biology (Clifton, N.J.). 2021 Mar 27


    PMID: 33772459

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