Maturation stage enamel defects in Odontogenesis-associated phosphoprotein (Odaph) deficient mice.

Mutation in Odontogenesis-associated phosphoprotein (ODAPH) has been reported to cause recessive hypomineralized amelogenesis imperfecta (AI) in human. However, the exact role of ODAPH in amelogenesis is still unknown. ODAPH was identified as a novel constituent of the atypical basal lamina located at the interface between maturation ameloblasts and the enamel by dual immunofluorescence staining of ODAPH and LAMC2. Odaph knockout mice were generated to explore the function of ODAPH in amelogenesis. Odaph-/- mice teeth showed severely attrition and reduced enamel mineralization. Histological analysis showed from transition or early-maturation stage, ameloblasts were rapidly shortened, lost cell polarity, and exhibited cell pathology. Abundant enamel matrix marked by amelogenin was retained. Temporary cyst-like structures were formed between flattened epithelial cells and the enamel from maturation stage to eruption. The integrity of the atypical basal lamina was impaired indicated by the reduced diffuse expression of LAMC2 and AMTN. The expression of maturation stage related genes of Amtn, Klk4, Integrinβ6 and Slc24a4 were significantly decreased. Our results suggested Odaph played vital roles during amelogenesis by maintaining the integrity of the atypical basal lamina in maturation stage, which may contribute to a better understanding of the pathophysiology of human AI. © 2021 American Association of Anatomists.

Citation

Yikang Ji, Cong Li, Yuan Tian, Yan Gao, Zhiheng Dong, Lili Xiang, Zhenzhen Xu, Yuguang Gao, Li Zhang. Maturation stage enamel defects in Odontogenesis-associated phosphoprotein (Odaph) deficient mice. Developmental dynamics : an official publication of the American Association of Anatomists. 2021 Oct;250(10):1505-1517

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PMID: 33772937

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