Identification of cancer driver genes using Sleeping Beauty transposon mutagenesis.

Haruna Takeda, Nancy A Jenkins, Neal G Copeland

Cancer science 2021 Jun

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Cancer genome sequencing studies have identified driver genes for a variety of different cancers and helped to understand the genetic landscape of human cancer. It is still challenging, however, to identify cancer driver genes with confidence simply from genetic data alone. In vivo forward genetic screens using Sleeping Beauty (SB) transposon mutagenesis provides another powerful genetic tool for identifying candidate cancer driver genes in wild-type and sensitized mouse tumors. By comparing cancer driver genes identified in human and mouse tumors, cancer driver genes can be identified with additional confidence based upon comparative oncogenomics. This review describes how SB mutagenesis works in mice and focuses on studies that have identified cancer driver genes in the mouse gastrointestinal tract. © 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Citation

Haruna Takeda, Nancy A Jenkins, Neal G Copeland. Identification of cancer driver genes using Sleeping Beauty transposon mutagenesis. Cancer science. 2021 Jun;112(6):2089-2096