The diversity of ubiquitin modifications is immense. A protein can be monoubiquitylated, multi-monoubiquitylated, and polyubiquitylated with chains varying in size and shape. Ubiquitin itself can be adorned with other ubiquitin-like proteins and smaller functional groups. Considering different combinations of post-translational modifications can give rise to distinct biological outcomes, characterizing ubiquitylated proteoforms of a given protein is paramount. In this Opinion, we review recent advances in detecting and quantifying various ubiquitin proteoforms using mass spectrometry. Copyright © 2021 Elsevier Ltd. All rights reserved.
Kirandeep K Deol, Eric R Strieter. The ubiquitin proteoform problem. Current opinion in chemical biology. 2021 Aug;63:95-104
PMID: 33813043
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