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    The complete mitochondrial genome of Brachionus rubens was sequenced using primers design, clone culture, DNA extraction, LONG-PCR amplification, purification and clone sequencing. We found that it is composed of two circular chromosomes, designated mtDNA I (11,398 bp) and mtDNA II (12,820 bp). The gene content of the B. rubens mitochondrial genome was similar to that of the previously reported mitochondrial genome of B. plicatilis. It contained 22 tRNA genes, 2 rRNA genes and 12 protein-coding genes (PCGs). Four of the 12 PCGs had an incomplete stop codons, TA(cob, atp6, nd3)or T(cox3). The A + T content of B. rubens mitochondrial genome was apparently higher (mtDNA-I 70.2% and mtDNA II 70.4%) than that of the mitochondrial genome of B. plicatilis (mtDNA-I 63.9% and mtDNA-II 62.9%). © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.


    Yi Zhang, Hai Ying Zhang, Jin Jin Yan, Meng Ning Xia, Ying Long Liu, Yan Mei Lu, Yun Zhi Yan, Shuang Huai Cheng. Complete mitochondrial genome of Brachionus rubens from wuhu, China (Rotifera, Brachionidae). Mitochondrial DNA. Part B, Resources. 2021 Mar 26;6(3):1194-1196

    PMID: 33829085

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